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Please use this identifier to cite or link to this item: http://hdl.handle.net/10386/526

Title: Biological activity analysis of the crude extract of the Senna species : structure elucidation of a compound with antioxidant activity
Authors: Gololo, Sechene Stanley
Advisors: Mampuru, Leseilane J.
Other Contributors: Eloff, Kobus
Masoko, Peter
Mokgotho, Matlou P.
Keywords: Senna species
Issue Date: 2008
Publisher: University of Limpopo
Abstract: Senna species, a member of the Fabaceae family (subfamily Caesalpinaceae), is widely used traditionally to treat a number of disease conditions such as sexually transmitted diseases and some forms of intestinal complications. In this study the roots of Senna species, collected from Zebediela region of the Limpopo province (R.S.A), were ground to a fine powder and extracted with acetone by cold/shaking extraction method. The phytochemical composition of the extract was then determined by thin layer chromatography (TLC). The chromatograms were visualised with vanillin-sulphuric acid and p-anisaldehyde reagents. The total phenolic content of the extract was determined by Folin-Ciocalteu method and expressed as TAE/g of dry plant material. The extract was assayed for the in vitro anticancer activity using Jurkat T cells. The antioxidant activity was evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay and the antibacterial activity determined by both bioautographic and the microtiter plate methods. The acetone extract of the roots of Senna species inhibited the growth of Jurkat T cells in a dose- and time-dependent manner. The extract was shown to possess free radical scavenging activity and antibacterial activity against Pseudomonas aeruginosa, Enterococcus faecalis, Escherichia coli and Staphylococcus aureus with MIC values of 0.16, 0.078, 0.078 and 0.16 mg/ml, respectively. A compound with free radical scavenging activity was isolated from the acetone extract of the roots of Senna species through bioassay-guided fractionation. The isolated compound was identified as 1, 3-diphenol-2-propen-1-one. Thus, the study has systematically shown the biological activity of the roots of Senna species and the isolation and identification of the bioactive compound.
Description: Thesis (M.Sc.) --University of Limpopo, 2008
URI: http://hdl.handle.net/10386/526
Library of Congress Subject Headings: Ethnobotany -- South Africa
Medicinal plants
Appears in Collections:Theses and Dissertations (Biochemistry)

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